Western blot analysis of extracts of various cell lines, using PYGM antibody (A9392) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||PYGM Rabbit pAb|
|Description:||This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PYGM (NP_005600.1).|
Help & Support