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Western blot analysis of extracts of HeLa cells, using PRPF18 antibody (A12817) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PRPF18 Rabbit pAb
Catalog No：AB-A12817
Manufacturer：ABclonal
Synonyms：PRPF18;PRP18;hPrp18
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | PRPF18 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1). |
| Gene ID: | 8559 |
| Swiss prot: | Q99633 |
| Molecular weight: | 5kDa/39kDa |
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