Western blot analysis of extracts of various cell lines, using PPIG antibody (A0429) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
|Product Name:||PPIG Rabbit pAb|
|Description:||PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PPIG (NP_004783.2).|
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