Western blot analysis of extracts of HUVEC cells, using Phospho-EGFR-Y998 antibody (AP0024) at 1:1000 dilution. HUVEC cells were treated by EGF.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
|Product Name:||Phospho-EGFR-Y998 Rabbit pAb|
|Description:||The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.|
|Immunogen:||A synthetic phosphorylated peptide around Y998 of human EGFR (NP_005219.2).|
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