Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 pAb (AP0019) at 1:2000 dilution or CREB1 antibody (A11064).HeLa cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-CREB1-S133 pAb (AP0019). Western blot was performed from the immunoprecipitate using Phospho-CREB1-S133 pAb (AP0019) at a dilition of 1:1000. 293T cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.
|Product Name:||Phospho-CREB1-S133 Rabbit pAb|
|Description:||This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.|
|Immunogen:||A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).|
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