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Western blot analysis of extracts of various cell lines, using PHF5A antibody (A12678) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
PHF5A Rabbit pAb
Catalog No：AB-A12678
Manufacturer：ABclonal
Synonyms：PHF5A;INI;Rds3;SAP14b;SF3B7;SF3b14b;bK223H9.2
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | PHF5A Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron’s branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PHF5A (NP_116147.1). |
| Gene ID: | 84844 |
| Swiss prot: | Q7RTV0 |
| Molecular weight: | 12kDa |
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