Western blot analysis of extracts of various cell lines, using PGK2 antibody (A12952) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
PGK2 Rabbit pAb
Catalog No:AB-A12952
Manufacturer:ABclonal
Synonyms:PGK2;HEL-S-272;PGKB;PGKPS;dJ417L20.2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€100,00 – €370,00
Product Name: | PGK2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2). |
Gene ID: | 5232 |
Swiss prot: | P07205 |
Molecular weight: | 44kDa |
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