Western blot analysis of extracts of various cell lines, using PELI3 Antibody (A9999) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
|Product Name:||PELI3 Rabbit pAb|
|Description:||The protein encoded by this gene is a scaffold protein and an intermediate signaling protein in the innate immune response pathway. The encoded protein helps transmit the immune response signal from Toll-like receptors to IRAK1/TRAF6 complexes. Several transcript variants encoding different isoforms have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PELI3 (NP_659502.2).|
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