Western blot analysis of extracts of various cell lines, using PBEF / NAMPT antibody (A0256) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
|Product Name:||PBEF / NAMPT Rabbit pAb|
|Description:||This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PBEF / NAMPT (NP_005737.1).|
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