Western blot analysis of extracts of various cell lines, using PABPC5 antibody (A9997) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||PABPC5 Rabbit pAb|
|Description:||This gene encodes a protein that binds to the polyA tail found at the 3′ end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PABPC5 (NP_543022.1).|
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