Western blot analysis of extracts of various cell lines, using NUP153 antibody (A8970) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
NUP153 Rabbit pAb
Catalog No:AB-A8970
Manufacturer:ABclonal
Synonyms:NUP153;HNUP153;N153
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€245,00 – €370,00
Product Name: | NUP153 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. |
Immunogen: | A synthetic peptide of human NUP153 |
Gene ID: | 9972 |
Swiss prot: | P49790 |
Molecular weight: | 149kDa/153kDa/157kDa |
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