Western blot analysis of extracts of various cell lines, using NUMB antibody (A9352) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
|Product Name:||NUMB Rabbit pAb|
|Description:||The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3).|
Help & Support