Western blot analysis of extracts of various cell lines, using NDUFA10 antibody (A10123) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
|Product Name:||NDUFA10 Rabbit pAb|
|Description:||The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human NDUFA10 (NP_004535.1).|
Help & Support