Western blot analysis of extracts of various cell lines, using NAGK antibody (A9070) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
|Product Name:||NAGK Rabbit pAb|
|Description:||This gene encodes a member of the N-acetylhexosamine kinase family. The encoded protein catalyzes the conversion of N-acetyl-D-glucosamine to N-acetyl-D-glucosamine 6-phosphate, and is the major mammalian enzyme which recovers amino sugars.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 50-210 of human NAGK (NP_060037.3).|
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