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Western blot analysis of extracts of various cell lines, using MRPS25 Rabbit pAb (A9083) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
MRPS25 Rabbit pAb
Catalog No：AB-A9083
Manufacturer：ABclonal
Synonyms：MRPS25;MRP-S25;RPMS25
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | MRPS25 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant protein of human MRPS25. |
| Gene ID: | 64432 |
| Swiss prot: | P82663 |
| Molecular weight: | 14kDa/16kDa/20kDa |
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