Western blot analysis of extracts of various cell lines, using MAN2A1 antibody (A10227) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
|Product Name:||MAN2A1 Rabbit pAb|
|Description:||This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 865-1144 of human MAN2A1 (NP_002363.2).|
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