Western blot analysis of extracts of various cell lines, using KEL antibody (A10116) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 20s.
|Product Name:||KEL Rabbit pAb|
|Description:||This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 70-310 of human KEL (NP_000411.1).|
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