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Western blot analysis of extracts of various cell lines, using Kaiso/Kaiso/ZBTB33 antibody (A12900) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Kaiso/ZBTB33 Rabbit pAb
Catalog No：AB-A12900
Manufacturer：ABclonal
Synonyms：ZBTB33;ZNF-kaiso;ZNF348
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | Kaiso/ZBTB33 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso/Kaiso/ZBTB33 (NP_006768.1). |
| Gene ID: | 10009 |
| Swiss prot: | Q86T24 |
| Molecular weight: | 74kDa |
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