Western blot analysis of extracts of MCF-7 cells, using ITK antibody (A12682) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||ITK Rabbit pAb|
|Description:||This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 450-620 of human ITK (NP_005537.3).|
Help & Support