Western blot analysis of extracts of RAW264.7 cells, using iNOS antibody (A0312) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
|Product Name:||iNOS Rabbit pAb|
|Description:||Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human iNOS (NP_000616.3).|
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