Western blot analysis of extracts of various cell lines, using IKKβ antibody (A0714) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
|Product Name:||IKKβ Rabbit pAb|
|Description:||The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 517-756 of human IKKβ (NP_001547.1).|
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