Western blot analysis of extracts of various cell lines, using IKKα antibody (A0422) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
|Product Name:||IKKα Rabbit pAb|
|Description:||This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 516-745 of human IKKα (NP_001269.3).|
Help & Support