Western blot analysis of extracts of various cell lines, using IKBKAP antibody (A10127) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||IKBKAP Rabbit pAb|
|Description:||The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).|
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