Western blot analysis of extracts of various cell lines, using IFT74 antibody (A12672) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||IFT74 Rabbit pAb|
|Description:||This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis–frontotemporal dementia and Bardet-Biedl Syndrome. [provided by RefSeq, Mar 2017]|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human IFT74 (NP_001092694.1).|
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