Western blot analysis of extracts of various cell lines, using HYI antibody (A12806) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
|Product Name:||HYI Rabbit pAb|
|Description:||This gene encodes a putative hydroxypyruvate isomerase, which likely catalyzes the conversion of hydroxypyruvate to 2-hydroxy-3-oxopropanoate, and may be involved in carbohydrate transport and metabolism. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 80-260 of human HYI (NP_001177809.1).|
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