Western blot analysis of extracts of various cell lines, using HSP27/HSP27/HSPB1 antibody (A0240) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||HSP27/HSPB1 Rabbit pAb|
|Description:||The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSP27/HSP27/HSPB1 (NP_001531.1).|
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