Western blot analysis of extracts of Rat liver, using Hemoglobin subunit alpha (HBA1) (HBA1) Rabbit mAb (A9293) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
|Product Name:||Hemoglobin subunit alpha (HBA1) Rabbit mAb|
|Description:||The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta – pseudozeta – mu – pseudoalpha-1 – alpha-2 – alpha-1 – theta – 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]|
|Immunogen:||A synthesized peptide derived from human Hemoglobin subunit alpha (HBA1) (HBA1)|
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