Western blot analysis of extracts of various cell lines, using HACE1 antibody (A9593) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||HACE1 Rabbit pAb|
|Description:||This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm’s tumor.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2).|
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