Western blot analysis of extracts of various cell lines, using GUCY1A3 antibody (A12710) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
|Product Name:||GUCY1A3 Rabbit pAb|
|Description:||Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3′,5′-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GUCY1A3 (NP_000847.2).|
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