Western blot analysis of extracts of various cell lines, using GSK3α antibody (A0645) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||GSK3α Rabbit pAb|
|Description:||This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer’s disease.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 409-483 of human GSK3α (NP_063937.2).|
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