Western blot analysis of extracts of various cell lines, using GJB4 Antibody (A9888) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||GJB4 Rabbit pAb|
|Description:||This gene encodes a transmembrane connexin protein that is a component of gap junctions. Mutations in this gene have been associated with erythrokeratodermia variabilis, progressive symmetric erythrokeratoderma and hearing impairment.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GJB4 (NP_694944.1).|
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