Western blot analysis of extracts of various cell lines, using FoxO3a antibody (A0102) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
|Product Name:||FoxO3a Rabbit pAb|
|Description:||This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FoxO3a (NP_062714.1).|
Help & Support