Western blot analysis of extracts of various cell lines, using FKRP antibody (A10194) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
|Product Name:||FKRP Rabbit pAb|
|Description:||This gene encodes a protein which is targeted to the medial Golgi apparatus and is necessary for posttranslational modification of dystroglycan. Mutations in this gene have been associated with congenital muscular dystrophy, mental retardation, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 30-220 of human FKRP (NP_077277.1).|
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