Western blot analysis of extracts of various cell lines, using FDX1 antibody (A9815) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
|Product Name:||FDX1 Rabbit pAb|
|Description:||This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human FDX1 (NP_004100.1).|
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