Western blot analysis of extracts of various cell lines, using ERCC3 antibody (A12702) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
|Product Name:||ERCC3 Rabbit pAb|
|Description:||This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne’s syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 512-782 of human ERCC3 (NP_000113.1).|
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