Western blot analysis of extracts of various cell lines, using ERC1 antibody (A9509) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
|Product Name:||ERC1 Rabbit pAb|
|Description:||The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13) in thyroid papillary carcinoma. Alternative splicing results in multiple transcript variants.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 700-850 of human ERC1 (NP_829884.1).|
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