Western blot analysis of extracts of various cell lines, using ENC1 antibody (A10257) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 40s.
|Product Name:||ENC1 Rabbit pAb|
|Description:||This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ENC1 (NP_003624.1).|
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