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Western blot analysis of extracts of various cell lines, using E2F3 antibody (A8811) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
E2F3 Rabbit pAb
Catalog No：AB-A8811
Manufacturer：ABclonal
Synonyms：E2F3;E2F-3
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | E2F3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human E2F3 (NP_001230005.1). |
| Gene ID: | 1871 |
| Swiss prot: | O00716 |
| Molecular weight: | 37kDa/49kDa |
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