Western blot analysis of extracts of various cell lines, using Desminmin antibody (A0699) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
|Product Name:||Desmin Rabbit pAb|
|Description:||This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 121-470 of human Desminmin (NP_001918.3).|
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