Western blot analysis of extracts of C2C12 cells, using DDIT3 / CHOP antibody (A0221) at 1:1000 dilution.C2C12 cells were treated by tunicamycin (2 μg/ml) for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||DDIT3 / CHOP Rabbit pAb|
|Description:||This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 / CHOP (NP_004074.2).|
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