Western blot analysis of extracts of various cell lines, using Cyclin K Antibody (A10261) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
|Product Name:||Cyclin K Rabbit pAb|
|Description:||The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human Cyclin K (NP_001092872.1).|
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