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Western blot analysis of extracts of 293T cells, using CTCF antibody (A13272) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human lung cancer using CTCF Rabbit pAb (A13272) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using CTCF antibody (A13272) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (A13272) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (A13272) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (A13272) at dilution of 1:200 (40x lens).
CTCF Rabbit pAb
Catalog No：AB-A13272
Manufacturer：ABclonal
Synonyms：CTCF;MRD21;CTCF
Applications：WB,IHC,IF,IP,ChIP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€100,00 – €370,00
| Product Name: | CTCF Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms’ tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1). |
| Gene ID: | 10664 |
| Swiss prot: | P49711 |
| Molecular weight: | 45kDa/82kDa |
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