Western blot analysis of extracts of various cell lines, using CRK/CRK/CRKII Rabbit mAb (A9577) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
CRK/CRKII Rabbit mAb
Catalog No:AB-A9577
Manufacturer:ABclonal
Synonyms:CRKII, p38
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€125,00 – €485,00
Product Name: | CRK/CRKII Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human CRK/CRK/CRKII |
Gene ID: | 1398 |
Swiss prot: | P46108 |
Molecular weight: | 38kDa |
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