Western blot analysis of extracts of various cell lines, using cIAP1 antibody (A0866) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
|Product Name:||cIAP1 Rabbit pAb|
|Description:||The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human cIAP1 (NP_001157.1).|
Help & Support