Western blot analysis of extracts of various cell lines, using CHST6 antibody (A10229) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
|Product Name:||CHST6 Rabbit pAb|
|Description:||The protein encoded by this gene is an enzyme that catalyzes the transfer of a sulfate group to the GlcNAc residues of keratan. Keratan sulfate helps maintain corneal transparency. Defects in this gene are a cause of macular corneal dystrophy (MCD).|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 196-395 of human CHST6 (NP_067628.1).|
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