Western blot analysis of extracts of K561 cells, using CD40L antibody (A13002) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
|Product Name:||CD40L Rabbit pAb|
|Description:||The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.|
|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 79-129 of human CD40L (NP_000065.1).|
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