Western blot analysis of extracts of various cell lines, using c-Jun antibody (A0246) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
|Product Name:||c-Jun Rabbit pAb|
|Description:||This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).|
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