Western blot analysis of extracts of various cell lines, using B3GALTL antibody (A9014) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
|Product Name:||B3GALTL Rabbit pAb|
|Description:||The protein encoded by this gene is a beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins. The encoded protein is a type II membrane protein. Defects in this gene are a cause of Peters-plus syndrome (PPS).|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 259-498 of human B3GALTL (NP_919299.3).|
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