Western blot analysis of extracts of various cell lines, using AGK antibody (A9976) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
|Product Name:||AGK Rabbit pAb|
|Description:||The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10.|
|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK (NP_060708.1).|
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