DNA LIBRARY PREPARATION

  • Rapid DNA Lib Prep kit
  • Full DNA Adapter Kit
  • Rapid Plus DNA Lib Prep kit
  • Rapid CE DNA Lib Prep kit
  • Full DNA Adapter Kit
  • Truncated DNA Adapter Kit

RNA library preparation

  • ABclonal First Strand Synthesis Module
  • Stranded mRNA-seq Lib Prep kit for illumina
  • mRNA-seq Lib Prep Kit
  • ABclonal First Strand Synthesis Module
  • ABclonal Second Strand Synthesis Module
  • ABclonal Whole RNA-seq Lib Prep kit
  • ABclonal rRNA Depletion Module (H/M/R)

Data Display

mRNA-seq Lib Prep Kit for Illumina (AB-RK20302)

Rapid DNA Lib Prep kit (AB-RK20301)

Featured Publications

    References for Rapid DNA Lib Prep kit (AB-RK20200)

          Product: Rapid DNA Lib Prep kit
          Journal: Frontiers in Genetics
          Application: PCR
          Species: Homo sapiens
          PMID: 32180799
          Title: Fragment Enrichment of Circulating Tumor DNA With Low-Frequency Mutations 

          Product: Rapid DNA Lib Prep kit
          Journal: Epilepsia
          Application: PCR
          Species: Homo sapiens
          PMID: 32216069
          Title: Somatic variants in new candidate genes identified in focal cortical dysplasia type II 

          Product: Rapid DNA Lib Prep kit
          Journal: Frontiers in Microbiology
          Application:
          Species: klebsiella pneumoniae
          PMID: 31849905
          Title: A Novel Polysaccharide Depolymerase Encoded by the Phage SH-KP152226 Confers Specific Activity Against Multidrug-Resistant Klebsiella pneumoniae via Biofilm Degradation. 

    References for ABclonal First Strand Synthesis Module (AB-RK20353)

          Product: ABclonal First Strand Synthesis Module
         Journal: Front Physiol
         Application: qRT-PCR
         Species: Rattus norvegicus
         PMID: 33613300
         Title: Matrine Protects Cardiomyocytes Against Hyperglycemic Stress by Promoting Mitofusin 2-Induced Mitochondrial Fusion 

    NGS Lib Prep Kits

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    Catalog No.: AB-RK20348
    Sequencer: Illumina

    ABclonal
    From €255
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    Catalog No.: AB-RK20303

    Sequencer: Illumina

    Suitable for Illumina sequencing platforms;
    The rRNA depletion module (H/M/R) is mainly for human, mouse and rat total RNA, which can effectively remove cytoplasmic rRNA from total RNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial rRNA (containing 12S rRNA and 16S rRNA).
    This kit is not only suit for complete RNA samples with good integrity, but also for RNA samples that have been degraded, such as FFPE RNA. The total amount of input RNA is 10 ng to 1 ug.
    Stranded mRNA Lib Prep method is recommended as a follow strategy, Whole RNA-seq data can be used to analyze all mRNAs except ribosomes as well as some long-chain non-coding RNAs, stranded Library can quantify gene expression and RNA species more accurately, and more fully understand gene structure.
    The kit contains an RNA truncated adapter, uses RNA index primer for PCR to add a unique index to each sample. Truncated adapter has higher connection efficiency than full length adapter and can reduce the generation of Adapter dimer;
    ABclonal Whole RNA-seq Lib Prep kit for illumine contains rRNA depletion module (H/M/R), First Strand synthesis module(stranded),Second cDNA synthesis module(stranded), DNA Lib Prep module with UDG and ABclonal RNA index primer. Reagents and enzymes for Whole RNA-seq Library preparation are all contained.
    Each reagent in the kit has undergone rigorous quality control, and each lot of kits has been verified by Lib preparation and sequencing, ensuring stable quality performance of each lot of kits.

    The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
    Components

    Box Module Name and color 24 Reactions (RK20303M) 96 Reactions (RK20303L)
    Box-1 rRNA Depletion Module (H/M/R)(RK20348) Probe Hybridization Buffer 48 μL 192 μL
    rRNA Probe Mix(H/M/R) 24 μL 96 μL
    RNase H 48 μL 192 μL
    10× RNase H Buffer 48 μL 192 μL
    DNase I 60 μL 240 μL
    10× DNase I Buffer 120 μL 480 μL
    Box-2 First StrandSynthesis Module (Stranded) (RK20342) 2X Frag/Elute Buffer 264 μL 1056 μL
    RT Strand Specificity Reagent 192 μl 768 μl
    First Strand Synthesis Enzyme Mix 48 μl 192 μl
    Second Strand Synthesis Module (Stranded) (RK20343) Second Strand Synthesis Reaction Buffer with dUTP 192 μl 768 μl
    Second Strand Synthesis Enzyme Mix 96 μl 384 μl
    Nuclease-free water 2 ml 8 ml
    DNA Lib Prep Module with UDG (RK20344) End Prep Buffer 240 μl 960 μl
    End Prep Enzyme Mix 72 μl 288 μl
    Ligation Buffer 396 μl 1584 μl
    Ligase Mix 72 μl 288 μl
    2X PCR Mix 600 μl 2×1200 μl
    UDG Enzyme 12 μl 48 μl
    Low-EDTA TE 2.5 ml 10 ml
    Box-3 RNA Adapter Module 24 Indices (RK20345) RNA Truncated Adapter 60 μl 240 μl
    RNA Universal Primer 60 μl 240 μl
    RNA Index Primer 2.5 μl* 10 μl*
    ABclonal
    From €1.080
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    Catalog No.: AB-RK20346
    Sequencer: Illumina

    ABclonal Second Strand Synthesis Module (AB-RK20346) generates a double-stranded cDNA product using the product from ABclonal First Strand cDNA Synthesis Module (AB-RK20342 or AB-RK20353) as a template for second strand cDNA synthesis.

    ABclonal Second Strand Synthesis Module (RK20346) generates a double-stranded cDNA product using the product from ABclonal First Strand cDNA Synthesis Module (RK20342 or RK20353) as a template for second strand cDNA synthesis.
    Component 24RXN (RK20346M) 96RXN (RK20346L)
    Second Strand Synthesis Reaction Buffer 192μL 768 μL
    Second Strand Synthesis Enzyme Mix 96μL 384 μL
    Nuclease-free Water 2mL 8mL
    Required Materials not Included First strand cDNA product, prepared using ABclonal First Strand Synthesis Module (AB-RK20342 or AB-RK20353)
    Magnetic stand
    80% ethanol (freshly prepared)
    200 μL PCR tube
    Agencourt AMPure XP beads
    Thermocycler
    Pipette
    ABclonal
    From €330
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    Catalog No.: AB-RK20342
    Sequencer: Illumina

    ABclonal First Strand Synthesis Module (Stranded) (RK20342) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product.

    ABclonal First Strand Synthesis Module (Stranded) (RK20342) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Strand Specificity Reagent in this module contains Actinomycin D to improve strand specificity and can be used with ABclonal Second Strand Synthesis Module (Stranded) (RK20343) to make strand-specific RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
    Component 24RXN (RK20342M) 96RXN (RK20342L)
    2×Frag/Elute Buffer 132μL 528 μL
    RT Strand Specificity Reagent 192μL 768 μL
    First Strand Synthesis Enzyme Mix 48μL 192 μL
    Required Materials not Included Poly(A)RNA, prepared using ABclonal Poly(A) mRNA Purification Module (AB-RK20341)
    rRNA depleted RNA, prepared using ABclonal rRNA Depletion Module (H/M/R) (AB-RK20348)
    200μL PCR tube
    Thermocycler
    Pipette
    ABclonal
    From €130
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    Catalog No.: AB-RK20294
    Sequencer: Illumina

    ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D (48 indices) is ideally suited for sample preparation for next-generation sequencing on the illumina platform.

    ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D (48 indices) is ideally suited for sample preparation for next-generation sequencing on the illumina platform. Truncated DNA adapters have higher DNA ligation efficiency, lower or no adapter-dimers than full-length Y-shape DNA adapters. ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D box contains truncated adapter, universal PCR primer and 48 PCR index oligos. When using truncated DNA adapters, a minimum number of PCR amplification cycles (2-3) may be required to complete adapter sequences required for hybridization of the templates to the flowcell surface, and for annealing of sequencing primers. The kit can NOT be used to perform PCR-free DNA library preparation. ABclonal Truncated DNA Adapter Kit for Illumina Set_C/Set_D (48 indices) can be used in the ABclonal Rapid DNA Lib Prep Kit and ABclonal StepWise DNA Lib Prep Kit.
    Name Index Volume Name Index volume
    Truncated Adapter - 600μL Universal PCR Primer - 600μL
    PCR Index 1 TTACCGAC 12.5μL PCR Index 25 TTCCAGGT 12.5μL
    PCR Index 2 AGTGACCT 12.5μL PCR Index 26 TCATCTCC 12.5μL
    PCR Index 3 TCGGATTC 12.5μL PCR Index 27 GAGAGTAC 12.5μL
    PCR Index 4 CAAGGTAC 12.5μL PCR Index 28 GTCGTTAC 12.5μL
    PCR Index 5 TCCTCATG 12.5μL PCR Index 29 GGAGGAAT 12.5μL
    PCR Index 6 GTCAGTCA 12.5μL PCR Index 30 AGGAACAC 12.5μL
    PCR Index 7 CGAATACG 12.5μL PCR Index 31 CAGTGCTT 12.5μL
    PCR Index 8 TCTAGGAG 12.5μL PCR Index 32 CTTGCTAG 12.5μL
    PCR Index 9 CGCAACTA 12.5μL PCR Index 33 TGGAAGCA 12.5μL
    PCR Index 10 CGTATCTC 12.5μL PCR Index 34 AGCTAAGC 12.5μL
    PCR Index 11 GTACACCT 12.5μL PCR Index 35 GAACGGTT 12.5μL
    PCR Index 12 CGGCATTA 12.5μL PCR Index 36 GGAATGTC 12.5μL
    PCR Index 13 TCGTCTGA 12.5μL PCR Index 37 TACGGTCT 12.5μL
    PCR Index 14 AGCCTATC 12.5μL PCR Index 38 CCAGTATC 12.5μL
    PCR Index 15 CTGTACCA 12.5μL PCR Index 39 TCTACGCA 12.5μL
    PCR Index 16 AGACCTTG 12.5μL PCR Index 40 GTAACCGA 12.5μL
    PCR Index 17 AGGATAGC 12.5μL PCR Index 41 GACGTCAT 12.5μL
    PCR Index 18 CCTTCCAT 12.5μL PCR Index 42 CTTACAGC 12.5μL
    PCR Index 19 GTCCTTGA 12.5μL PCR Index 43 TCCATTGC 12.5μL
    PCR Index 20 TGCGTAAC 12.5μL PCR Index 44 AGCGAGAT 12.5μL
    PCR Index 21 CACAGACT 12.5μL PCR Index 45 CAATAGCC 12.5μL
    PCR Index 22 TTACGTGC 12.5μL PCR Index 46 AAGACACC 12.5μL
    PCR Index 23 CCAAGGTT 12.5μL PCR Index 47 CCAGTTGA 12.5μL
    PCR Index 24 CACGCAAT 12.5μL PCR Index 48 TGGTGAAG 12.5μL

    ABclonal
    Regular price €960
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    Catalog No.: AB-RK20212
    Sequencer: Illumina

    The Rapid CE DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina® platforms. The entire protocol can be completed in 2 hours.

    The Rapid CE DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
    The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
    Tube name 8 RXN 24 RXN 96 RXN
    End Preparation End Prep Buffer 100 μl 260 μl 1000 μl
    End Prep Enzymes 30 μl 78 μl 300 μl
    Adaptor Ligation Ligase MM 165 μl 429 μl 1650 μl
    Ligase Mix 30 μl 78 μl 300 μl
    Low-EDTA TE Buffer 1000 μl 2 mL 5 mL
    Amplification 2X PCR Mix 250 μl 650 μl 2.5 mL
    10X PCR Primers 40 μl 120 μl 500 μl

    Required Materials not Included Mechanical or enzymatic methods for DNA fragmentation
    Multiplex adapters (cat. no. AB-RK20282, AB-RK20283, AB-RK20284, AB-RK20285, AB-RK20292, AB-RK20293, AB-RK20294, AB-RK20295) compatible with Illumina® platforms
    100% ethanol (ACS grade)
    Nuclease-free water
    PCR strip tubes or plates
    Magnetic stand
    Thermocycler
    AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. AB-A63880)
    Pipettes and multichannel pipettes
    Aerosol resistant pipette tips
    Microcentrifuge
    Vortex mixer
    Agilent Bioanalyzer or comparable method to assess the quality of DNA library
    ABclonal
    From €430
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    Catalog No.: AB-RK20208
    Sequencer: Illumina

    The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours.

    The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
    The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.
    The following workflow illustrates the processes of Rapid DNA Lib Prep Kit.

    Tube name 8 RXN 24 RXN 96 RXN
    End Preparation End Prep Buffer 70 μl 168 μl 700 μl
    End Prep Enzymes 30 μl 78 μl 300 μl
    Damaged DNA Repair Enzymes 30 μl 78 μl 300 μl
    Adaptor Ligation Ligation Buffer 300 μl 780 μl 3 ml
    Ligation Enzymes 100 μl 240 μl 1000 μl
    Amplification 2X PCR Master Mix 250 μl 650 μl 2.5 mL
    10X PCR Primers 50 μl 130 μl 500 μl
    Required Materials not Included Mechanical or enzymatic methods for DNA fragmentation
    Multiplex adapters (cat. no. AB-RK20282, AB-RK20283, AB-RK20284, AB-RK20285, AB-RK20292, AB-RK20293, AB-RK20294, AB-RK20295) compatible with Illumina® platforms
    100% ethanol (ACS grade)
    Nuclease-free water
    PCR strip tubes or plates
    Magnetic stand
    Thermocycler
    AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. AB-A63880)
    Pipettes and multichannel pipettes
    Aerosol resistant pipette tips
    Microcentrifuge
    Vortex mixer
    Agilent Bioanalyzer or comparable method to assess the quality of DNA library
    ABclonal
    From €450
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    Catalog No.: AB-RK20302
    Sequencer: Illumina

    The ABclonal mRNA-seq Lib Prep Kit for IlluminaR comprises four processing modules: Poly(A) mRNA Purification, First Strand Synthesis, Second cDNA Synthesis and DNA Library Preparation.

    The ABclonal mRNA-seq Lib Prep Kit for IlluminaR comprises four processing modules: Poly(A) mRNA Purification, First Strand Synthesis, Second cDNA Synthesis and DNA Library Preparation. The kit includes all of the enzymes and buffers for poly(A) mRNA enrichment and mRNA-seq library construction, from 10 - 1000 ng of total RNA isolated from a wide variety of eukaryotic species
    The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
    Box Module Name Tube Color & Name 24 Reactions (RK20302M) 96 Reactions (RK20302L)
    Box-1 Poly(A) mRNA Purification Module (RK20341) Oligo d(T)25 Capture Beads 480 μl 1920 μl
    mRNA Binding Buffer 12 ml 48 ml
    Washing Buffer 19.2 ml 76.8 ml
    Tris Buffer 1.2 ml 4.8 ml
    Box-2 First Strand Synthesis Module (RK20353) 2X Frag/Elute Buffer 144 μl 576 μl
    RT Reagent 192 μl 768 μl
    First Strand Synthesis Enzyme Mix 48 μl 192 μl
    Second Strand Synthesis Module (RK20346) Second Strand Synthesis Reaction Buffer 192 μl 768 μl
    Second Strand Synthesis Enzyme Mix 96 μl 384 μl
    Nuclease-free Water 2 ml 8 ml
    DNA Lib Prep Module (RK20347) End Prep Buffer 240 μl 960 μl
    End Prep Enzyme Mix 72 μl 288 μl
    Ligation Buffer 396 μl 1584 μl
    Ligase Mix 72 μl 288 μl
    2X PCR Mix 600 μl 2×1200 μl
    Low-EDTA TE 2.5 ml 10 ml
    Box-3 RNA Adapter Module (24 Indices)(RK20345) RNA Truncated Adapter 60 μl 240 μl
    RNA Universal Primer 60 μl 240 μl
    RNA Index Primer 2.5 μl* 10 μl*
    Required Materials not Included 100% ethanol (ACS grade)
    Nuclease-free water
    Nuclease-free PCR tubes or plates
    Magnetic stand
    Thermocycler
    AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. AB-A63880)
    Pipettes and multichannel pipettes
    Aerosol resistant pipette tips
    Microcentrifuge
    Vortex mixer
    Agilent Bioanalyzer or comparable method to assess the quality of stranded mRNA-seq library
    ABclonal
    From €618
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    Catalog No.: AB-RK20301
    Sequencer: Illumina

    Suitable for Illumina sequencing platforms; Suitable for samples: eukaryotic RNA samples such as animals, plants and fungi; Total RNA starting at 10ng-1μg, recommended RNA RIN value ≥7;

    Suitable for Illumina sequencing platforms;
    Suitable for samples: eukaryotic RNA samples such as animals, plants and fungi; Total RNA starting at 10ng-1μg, recommended RNA RIN value ≥7;
    The kit contains an RNA truncated adapter, uses RNA index primer for PCR to add a unique index to each sample. Truncated adapter has higher connection efficiency than full length adapter and can reduce the generation of Adapter dimer;
    Stranded mRNA-seq Lib Prep kit for illumina contains mRNA Capture module, First Strand synthesis module, Second cDNA synthesis module, DNA Lib Prep module and ABclonal RNA index primer. All buffers and enzymes required for mRNA Lib Prep are included.

    The kit labels Second strand cDNA with dUTP during the synthesis. The Second strand is hydrolyzed by UDG enzyme before PCR enrichment step, to make sure all the sequence data is from the First Strand cDNA, which retains the mRNA strand orientation, to reach the need for strand-specific mRNA-seq.
    Each reagent in the kit has undergone rigorous quality control, and each lot of kits has been verified by Lib preparation and sequencing, ensuring stable quality performance of each lot of kits.
    The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.
    Box Module Name Tube Color & Name 24 Reactions (RK20301M) 96 Reactions (RK20301L)
    Box-1 Poly(A) mRNA Purification Module (RK20341) Oligo d(T)25 Capture Beads 480 μl 1920 μl
    mRNA Binding Buffer 12 ml 48 ml
    Washing Buffer 19.2 ml 76.8 ml
    Tris Buffer 1.2 ml 4.8 ml
    Box-2 First Strand Synthesis Module (Stranded) (RK20342) 2X Frag/Elute Buffer 144 μl 576 μl
    RT Strand Specificity Reagent 192 μl 768 μl
    First Strand Synthesis Enzyme Mix 48 μl 192 μl
    Second Strand Synthesis Module (Stranded) (RK20343) Second Strand Synthesis Reaction Buffer with dUTP 192 μl 768 μl
    Second Strand Synthesis Enzyme Mix 96 μl 384 μl
    Nuclease-free Water 2 ml 8 ml
    DNA Lib Prep Module with UDG (RK20344) End Prep Buffer 240 μl 960 μl
    End Prep Enzyme Mix 72 μl 288 μl
    Ligation Buffer 396 μl 1584 μl
    Ligase Mix 72 μl 288 μl
    2X PCR Mix 600 μl 2×1200 μl
    UDG Enzyme 12 μl 48 μl
    Low-EDTA TE 2.5 ml 10 ml
    Box-3 RNA Adapter Module (24 Indices) (RK20345) RNA Truncated Adapter 60 μl 240 μl
    RNA Universal Primer 60 μl 240 μl
    RNA Index Primer 2.5 μl* 10 μl*
    Required Materials not Included 100% ethanol (ACS grade)
    Nuclease-free water
    Nuclease-free PCR tubes or plates
    Magnetic stand
    Thermocycler
    AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. AB-A63880)
    Pipettes and multichannel pipettes
    Aerosol resistant pipette tips
    Microcentrifuge
    Vortex mixer
    Agilent Bioanalyzer or comparable method to assess the quality of stranded mRNA-seq library
    ABclonal
    From €640
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    Catalog No.: AB-RK20293
    Sequencer: Illumina

    ABclonal Full DNA Adapter Kit for Illumina Set_D (48 indices) are ideally suited for sample preparation for next-generation sequencing on the illumina platform. Set_D box contains 48 adapters from 96-index adapter_49 to adapter_96.

    ABclonal Full DNA Adapter Kit for Illumina Set_D (48 indices) are ideally suited for sample preparation for next-generation sequencing on the illumina platform. Set_D box contains 48 adapters from 96-index adapter_49 to adapter_96. The kit can be used to perform PCR-free DNA library preparation, in which adapters contain the complete sequence required for hybridization of the templates to the flowcell surface, and for annealing of sequencing primers.
    Full DNA Adapter Kit for Illumina Set_D (48 indices) can be used in the ABclonal Rapid DNA Lib Prep Kit and ABclonal StepWise DNA Lib Prep Kit.
    Set_D adapters Index Volume Set_D adapters Index Volume
    Adapter_96-49 GATAGACA 12.5 μl Adapter_96-61 TATCAGCA 12.5 μl
    Adapter_96-50 GCCACATA 12.5 μl Adapter_96-62 TCCGTCTA 12.5 μl
    Adapter_96-51 GCGAGTAA 12.5 μl Adapter_96-63 TCTTCACA 12.5 μl
    Adapter_96-52 GCTAACGA 12.5 μl Adapter_96-64 TGAAGAGA 12.5 μl
    Adapter_96-53 GCTCGGTA 12.5 μl Adapter_96-65 TGGAACAA 12.5 μl
    Adapter_96-54 GGAGAACA 12.5 μl Adapter_96-66 TGGCTTCA 12.5 μl
    Adapter_96-55 GGTGCGAA 12.5 μl Adapter_96-67 TGGTGGTA 12.5 μl
    Adapter_96-56 GTACGCAA 12.5 μl Adapter_96-68 TTCACGCA 12.5 μl
    Adapter_96-57 GTCGTAGA 12.5 μl Adapter_96-69 AACTCACC 12.5 μl
    Adapter_96-58 GTCTGTCA 12.5 μl Adapter_96-70 AAGAGATC 12.5 μl
    Adapter_96-59 GTGTTCTA 12.5 μl Adapter_96-71 AAGGACAC 12.5 μl
    Adapter_96-60 TAGGATGA 12.5 μl Adapter_96-72 AATCCGTC 12.5 μl
    Adapter_96-73 AATGTTGC 12.5 μl Adapter_96-85 CCATCCTC 12.5 μl
    Adapter_96-74 ACACGACC 12.5 μl Adapter_96-86 CCGACAAC 12.5 μl
    Adapter_96-75 ACAGATTC 12.5 μl Adapter_96-87 CCTAATCC 12.5 μl
    Adapter_96-76 AGATGTAC 12.5 μl Adapter_96-88 CCTCTATC 12.5 μl
    Adapter_96-77 AGCACCTC 12.5 μl Adapter_96-89 CGACACAC 12.5 μl
    Adapter_96-78 AGCCATGC 12.5 μl Adapter_96-90 CGGATTGC 12.5 μl
    Adapter_96-79 AGGCTAAC 12.5 μl Adapter_96-91 CTAAGGTC 12.5 μl
    Adapter_96-80 ATAGCGAC 12.5 μl Adapter_96-92 GAACAGGC 12.5 μl
    Adapter_96-81 ATCATTCC 12.5 μl Adapter_96-93 GACAGTGC 12.5 μl
    Adapter_96-82 ATTGGCTC 12.5 μl Adapter_96-94 GAGTTAGC 12.5 μl
    Adapter_96-83 CAAGGAGC 12.5 μl Adapter_96-95 GATGAATC 12.5 μl
    Adapter_96-84 CACCTTAC 12.5 μl Adapter_96-96 GCCAAGAC 12.5 μl

     

    ABclonal
    Regular price €475
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    Catalog No.: AB-RK20353
    Sequencer: Illumina

    ABclonal First Strand Synthesis Module (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product.

    ABclonal First Strand Synthesis Module (Stranded) (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
    Component 24RXN (RK20353M) 96RXN (RK20353L)
    2×Frag/Elute Buffer 132μL 528 μL
    RT Reagent 192μL 768 μL
    First Strand Synthesis Enzyme Mix 48μL 192 μL
    Required Materials not Included Poly(A)RNA, prepared using ABclonal Poly(A) mRNA Purification Module (AB-RK20341)
    rRNA depleted RNA, prepared using ABclonal rRNA Depletion Module (H/M/R) (AB-RK20348)
    200μL PCR tube
    Thermocycler
    Pipette
    PMID: 33613300  Title: Matrine Protects Cardiomyocytes Against Hyperglycemic Stress by Promoting Mitofusin 2-Induced Mitochondrial Fusion 
    ABclonal
    From €175
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    Catalog No.: AB-RK20200
    Sequencer: Illumina

    The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina platforms. The entire protocol can be completed in 2 hours.

    Adavantages

    Input DNA amount: 1ng-1µg
    High DNA library conversion, and less PCR amplification cycles;
    Suitable for FFPE DNA and cfDNA
    Reduced PCR bias
    Lower cost per library

    The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
    Tube name 8 RXN 24 RXN 96 RXN
    End Preparation End Prep Buffer 100 μl 260 μl 1000 μl
    End Prep Enzymes 30 μl 78 μl 300 μl
    Adaptor Ligation Ligase MM 165 μl 429 μl 1650 μl
    Ligase Mix 30 μl 78 μl 300 μl
    Low-EDTA TE Buffer 1000 μl 2 mL 5 mL
    Amplification 2X PCR Master Mix 250 μl 650 μl 2.5 mL
    10X PCR Primers 40 μl 120 μl 500 μl
    Required Materials not Included Mechanical or enzymatic methods for DNA fragmentation
    Multiplex adapters (cat. no. AB-RK20282, AB-RK20283, AB-RK20284, AB-RK20285, AB-RK20292, AB-RK20293, AB-RK20294, AB-RK20295) compatible with Illumina(R) platforms
    100% ethanol (ACS grade)
    Nuclease-free water
    PCR strip tubes or plates
    Magnetic stand
    Thermocycler
    AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. AB-A63880)
    Pipettes and multichannel pipettes
    Aerosol resistant pipette tips
    Microcentrifuge
    Vortex mixer
    Agilent Bioanalyzer or comparable method to assess the quality of DNA library
    PMID: 32180799  Title: Fragment Enrichment of Circulating Tumor DNA With Low-Frequency Mutations  PMID: 32216069  Title: Somatic variants in new candidate genes identified in focal cortical dysplasia type II  PMID: 31849905  Title: A Novel Polysaccharide Depolymerase Encoded by the Phage SH-KP152226 Confers Specific Activity Against Multidrug-Resistant Klebsiella pneumoniae via Biofilm Degradation. 
    ABclonal
    From €440
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